PT  - JOURNAL ARTICLE
AU  - Mehmet U. Girgin
AU  - Nicolas Broguiere
AU  - Sylke Hoehnel
AU  - Nathalie Brandenberg
AU  - Bastien Mercier
AU  - Alfonso Martinez Arias
AU  - Matthias P. Lutolf
TI  - Bioengineered embryoids mimic post-implantation development <em>in vitro</em>
AID  - 10.1101/2021.01.10.426096
DP  - 2021 Jan 01
TA  - bioRxiv
PG  - 2021.01.10.426096
4099  - http://biorxiv.org/content/early/2021/01/10/2021.01.10.426096.short
4100  - http://biorxiv.org/content/early/2021/01/10/2021.01.10.426096.full
AB  - The difficulty of studying post-implantation development in mammals has sparked a flurry of activity to develop in vitro models, termed embryoids, based on self-organizing pluripotent stem cells. Previous approaches to derive embryoids either lack the physiological morphology and signaling interactions, or are not yet optimal for modeling post-gastrulation development. Here, we report a bioengineering-inspired approach aimed at addressing this gap. A high-throughput cell aggregation approach was employed to simultaneously coax mouse embryonic stem cells (ESCs) into hundreds of uniform epiblast-like (EPI) aggregates in a solid matrix-free manner. When co-cultured with mouse trophoblast stem cell (TSC) aggregates, the resulting hybrid structures initiate gastrulation-like events and undergo axial morphogenesis to yield structures, termed EpiTS embryoids, with a pronounced anterior development, including brain-like regions. We identify the presence of an epithelium in EPI aggregates as the major determinant for the axial morphogenesis and anterior development seen in EpiTS embryoids. Our results demonstrate the potential of EpiTS embryoids to study peri-gastrulation development in vitro.Competing Interest StatementThe EPFL has filed for patent protection on the microwell array technology applied herein, and S.H., N.B. and M.P.L. are named as inventors on those patents; S.H., N.B. and M.P.L. are shareholder in SUN bioscience SA, which is commercializing those patents.