TY - JOUR T1 - <em>In vivo</em> visualization of pig vagus nerve ‘vagotopy’ using ultrasound JF - bioRxiv DO - 10.1101/2020.12.24.424256 SP - 2020.12.24.424256 AU - Megan L. Settell AU - Maïsha Kasole AU - Aaron C. Skubal AU - Bruce E. Knudsen AU - Evan N. Nicolai AU - Chengwu Huang AU - Chenyun Zhou AU - James K. Trevathan AU - Aniruddha Upadhye AU - Chaitanya Kolluru AU - Andrew J. Shoffstall AU - Justin C. Williams AU - Aaron J. Suminski AU - Warren M. Grill AU - Nicole A. Pelot AU - Shigao Chen AU - Kip A. Ludwig Y1 - 2021/01/01 UR - http://biorxiv.org/content/early/2021/01/11/2020.12.24.424256.abstract N2 - Background Placement of the clinical vagus nerve stimulating cuff is a standard surgical procedure based on anatomical landmarks, with limited patient specificity in terms of fascicular organization or vagal anatomy. As such, the therapeutic effects are generally limited by unwanted side effects of neck muscle contractions, demonstrated by previous studies to result from stimulation of 1) motor fibers near the cuff in the superior laryngeal and 2) motor fibers within the cuff projecting to the recurrent laryngeal.Objective The use of patient-specific visualization of vagus nerve fascicular organization could better inform clinical cuff placement and improve clinical outcomes.Methods The viability of ultrasound, with the transducer in the surgical pocket, to visualize vagus nerve fascicular organization (i.e. vagotopy) was characterized in a pig model. Ultrasound images were matched to post-mortem histology to confirm the utility of ultrasound in identifying fascicular organization.Results High-resolution ultrasound accurately depicted the vagotopy of the pig vagus nerve intra-operatively, as confirmed via histology. The stereotypical pseudo-unipolar cell body aggregation at the nodose ganglion was identifiable, and these sensory afferent fascicular bundles were traced down the length of the vagus nerve. Additionally, the superior and recurrent laryngeal nerves were identified via ultrasound.Conclusions Intraoperative visualization of vagotopy and surrounding nerves using ultrasound is a novel approach to optimize stimulating cuff placement, avoid unwanted activation of motor nerve fibers implicated in off-target effects, and seed patient-specific models of vagal fiber activation to improve patient outcomes.Competing Interest StatementThe authors have declared no competing interest. ER -