RT Journal Article
SR Electronic
T1 Experimental and mathematical insights on the interactions between poliovirus and a defective interfering genome
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 2021.01.11.426198
DO 10.1101/2021.01.11.426198
A1 Yuta Shirogane
A1 Elsa Rousseau
A1 Jakub Voznica
A1 Yinghong Xiao
A1 Weiheng Su
A1 Adam Catching
A1 Zachary J. Whitfield
A1 Igor M. Rouzine
A1 Simone Bianco
A1 Raul Andino
YR 2021
UL http://biorxiv.org/content/early/2021/01/11/2021.01.11.426198.abstract
AB During replication, RNA viruses accumulate genome alterations, such as mutations and deletions. The interactions between individual variants can determine the fitness of the virus population and, thus, the outcome of infection. To investigate the effects of defective interfering genomes (DI) on wild-type (WT) poliovirus replication, we developed an ordinary differential equation model. We experimentally determined virus and DI replication during co-infection, and use these data to infer model parameters. Our model predicts, and our experimental measurements confirm, that DI replication and encapsidation are the most important determinants for the outcome of the competition. WT replication inversely correlates with DI replication. Our model predicts that genome replication and effective DI genome encapsidation are critical to effectively inhibit WT production, but an equilibrium can be established which enables WT to replicate, albeit to reduce levels.