RT Journal Article
SR Electronic
T1 A vacuolar hexose transport is required for xylem development in the inflorescence stem of Arabidopsis
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 2020.12.09.417345
DO 10.1101/2020.12.09.417345
A1 Emilie Aubry
A1 Beate Hoffmann
A1 Françoise Vilaine
A1 Françoise Gilard
A1 Patrick A.W. Klemens
A1 Florence Guérard
A1 Bertrand Gakière
A1 H. Ekkehard Neuhaus
A1 Catherine Bellini
A1 Sylvie Dinant
A1 Rozenn Le Hir
YR 2021
UL http://biorxiv.org/content/early/2021/01/12/2020.12.09.417345.abstract
AB In higher plants, the development of the vascular system is controlled by a complex network of transcription factors. However, how nutrient availability in the vascular cells affects their development remains to be addressed. At the cellular level, cytosolic sugar availability is regulated mainly by sugar exchanges at the tonoplast through active and/or facilitated transport. In Arabidopsis thaliana, among the tonoplastic transporters, SWEET16 and SWEET17 have been previously localized in the vascular system. Here, using a reverse genetic approach, we propose that sugar exchanges at the tonoplast, mediated by SWEET16, are important for xylem cell division as revealed in particular by the decreased number of xylem cells in the swt16 mutant and the expression of SWEET16 at the procambium-xylem boundary. In addition, we demonstrate that transport of hexoses mediated by SWEET16 and/or SWEET17 is required to sustain the formation of the xylem secondary cell wall. This result is in line with a defect in the xylem cell wall composition as measured by FTIR in the swt16swt17 double mutant and by upregulation of several genes involved in secondary cell wall synthesis. Our work therefore supports a model in which xylem development is partially dependent on the exchange of hexoses at the tonoplast of xylem-forming cells.Competing Interest StatementThe authors have declared no competing interest.