TY - JOUR T1 - Molecular architecture of the endocytic TPLATE complex JF - bioRxiv DO - 10.1101/2020.08.13.249276 SP - 2020.08.13.249276 AU - Klaas Yperman AU - Jie Wang AU - Dominique Eeckhout AU - Joanna Winkler AU - Lam Dai Vu AU - Michael Vandorpe AU - Peter Grones AU - Evelien Mylle AU - Michael Kraus AU - Romain Merceron AU - Jonah Nolf AU - Eliana Mor AU - Pieter De Bruyn AU - Remy Loris AU - Martin Potocký AU - Savvas N. Savvides AU - Bert De Rybel AU - Geert De Jaeger AU - Daniël Van Damme AU - Roman Pleskot Y1 - 2021/01/01 UR - http://biorxiv.org/content/early/2021/01/13/2020.08.13.249276.abstract N2 - Eukaryotic cells rely on endocytosis to regulate their plasma membrane proteome and lipidome. Most eukaryotic groups, except fungi and animals, have retained the evolutionary ancient TSET complex as endocytic regulator. Unlike other coatomer complexes, structural insight into TSET is lacking. Here, we reveal the molecular architecture of plant TSET (TPLATE complex/TPC) using an integrative structural approach. We identify crucial roles for specific TSET subunits in complex assembly and membrane interaction. Our data therefore generates novel insight into the differences between the hexameric TSET in Dictyostelium and the octameric TPC in plants. Structural elucidation of this ancient adaptor complex represents the missing piece in the coatomer-puzzle and vastly advances our functional as well as evolutionary insight into the process of endocytosis.One Sentence Summary An integrative structural approach reveals crucial roles of specific subunits in the plant TPLATE/TSET complex.Competing Interest StatementThe authors have declared no competing interest. ER -