TY - JOUR T1 - Biomaterials direct functional B cell response in a material specific manner JF - bioRxiv DO - 10.1101/2021.01.12.426347 SP - 2021.01.12.426347 AU - Erika M. Moore AU - David R. Maestas, Jr. AU - Chris C. Cherry AU - Jordan A. Garcia AU - Hannah Y. Comeau AU - Locke Davenport Huyer AU - Richard L. Blosser AU - Gedge D. Rosson AU - Jennifer H. Elisseeff Y1 - 2021/01/01 UR - http://biorxiv.org/content/early/2021/01/13/2021.01.12.426347.abstract N2 - B cells are an adaptive immune target of biomaterials development in vaccine research but despite their role in wound healing have not been studied in tissue engineering and regenerative medicine. We evaluated the B cell response to biomaterial scaffold materials implanted in a muscle wound; a biological extracellular matrix (ECM) and synthetic polyester polycaprolactone. In the local muscle tissue, small numbers of B cells are recruited in response to tissue injury and biomaterial implantation. ECM materials induced plasmablasts in lymph nodes and antigen presentation in the spleen while the synthetic PCL implants delayed B cell migration and induced an antigen presenting phenotype. In muMt− mice lacking B cells, the fibrotic response to the synthetic biomaterials decreased. Immunofluorescence confirmed antigen presenting B cells in fibrotic tissue surrounding silicone breast implants. In sum, the adaptive B cell immune response to biomaterial depends on composition and induces local, regional and systemic immunological changes.Competing Interest StatementJ.H.E. is an inventor on intellectual property related to biological scaffolds and inhibiting fibrosis. J.H.E. is a consultant to ACell, Unity Biotechnology and a founder of Aegeria. The authors declare no other competing interests. ER -