PT  - JOURNAL ARTICLE
AU  - Anh Hoang Le
AU  - Tamas Yelland
AU  - Nikki R. Paul
AU  - Loic Fort
AU  - Savvas Nikolaou
AU  - Shehab Ismail
AU  - Laura M. Machesky
TI  - CYRI-A regulates macropinocytic cup maturation and mediates integrin uptake, limiting invasive migration
AID  - 10.1101/2020.12.04.411645
DP  - 2021 Jan 01
TA  - bioRxiv
PG  - 2020.12.04.411645
4099  - http://biorxiv.org/content/early/2021/01/16/2020.12.04.411645.short
4100  - http://biorxiv.org/content/early/2021/01/16/2020.12.04.411645.full
AB  - The Scar/WAVE complex is the major driver of actin nucleation at the plasma membrane, resulting in lamellipodia and membrane ruffles. While lamellipodia aid migration, membrane ruffles can generate macropinosomes - cup-like structures - important for nutrient uptake and regulation of cell surface receptor levels. How macropinosomes are formed and the role of the actin machinery in their formation and resolution is still not well understood. Mammalian CYRI-B is a recently described negative regulator of the Scar/WAVE complex by RAC1 sequestration, but its other paralogue, CYRI-A has not been characterised. Here we implicate CYRI-A as a key regulator of macropinosome maturation and integrin internalisation from the cell surface. We find that CYRI-A is recruited to nascent macropinosomes in a transient but distinct burst, downstream of PIP3-mediated RAC1 activation and the initial burst of actin assembly driving cup formation, but upstream of internalisation and RAB5 recruitment to the macropinosome. Together, our data place CYRI-A as a local suppressor of actin dynamics, enabling the resolution of the macropinocytic cup. The failure of CYRI-depleted cells to resolve their macropinocytic cups results in reduced integrin a5b1 internalisation, leading to enhanced spreading, invasive behaviour and anchorage-independent 3D growth. We thus describe a new role for CYRI-A as a highly dynamic regulator of RAC1 activity at macropinosomes, modulating homeostasis of integrin surface presentation, with important functional consequences.Competing Interest StatementThe authors have declared no competing interest.