TY - JOUR T1 - A sexual dimorphic role of the <em>miR-465</em> cluster in placental development JF - bioRxiv DO - 10.1101/2021.01.16.426973 SP - 2021.01.16.426973 AU - Zhuqing Wang AU - Nan Meng AU - Yue Wang AU - Tong Zhou AU - Musheng Li AU - Shawn Wang AU - Sheng Chen AU - Huili Zheng AU - Shuangbo Kong AU - Haibin Wang AU - Wei Yan Y1 - 2021/01/01 UR - http://biorxiv.org/content/early/2021/01/17/2021.01.16.426973.abstract N2 - A sexually dimorphic role of miRNAs in placental development has never been reported. Here, we show that ablation of the miR-465 cluster caused selective degeneration of female conceptuses as early as embryonic day (E)8.5, leading to a male-biased sex ratio (60% males) among miR-465 KO mice. Given that the miR-465 cluster miRNAs were predominantly expressed in the extraembryonic tissue and ablation of these miRNAs led to dysregulation of numerous critical placental genes, our data strongly suggest the miR-465 cluster is required for full developmental potential of the female, but not the male, extraembryonic tissue/placenta.Competing Interest StatementThe authors have declared no competing interest.WTwild-typeKOknockoutMmaleFfemaleEmembryoExEextraembryonic tissuePlplacentaChchorionEPCectoplacental coreAlallantoisDedeciduaISHin situ hybridizationNSnot significantNnormal-looking conceptusesDdelayed/degenerating/degenerated conceptusesDEGsdifferentially expressed genesPCAprincipal component analysesGOGene ontologyXCIX chromosome inactivationTPMTranscript per million reads ER -