RT Journal Article
SR Electronic
T1 Aminooxadiazolyl kainic acid reveals that kainic acid receptors contribute to astrocytoma glutamate signaling
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 2021.01.16.426948
DO 10.1101/2021.01.16.426948
A1 Mitra Sadat Tabatabaee
A1 Zhenlin Tian
A1 Julien Gibon
A1 Frederic Menard
YR 2021
UL http://biorxiv.org/content/early/2021/01/18/2021.01.16.426948.abstract
AB The excitatory neurotransmitter glutamate triggers a Ca2+ rise and the extension of processes in astrocytes. Our results suggest that kainic acid receptors (KAR) can independently initiate glutamate signaling in astrocytoma U118-MG cells. The natural product kainic acid triggered glioexcitablity in cells and was inhibited by the KAR antagonist CNQX, but its activity was lower than glutamate on KARs. We created a new heteroaryl kainoid based on rational design: aminooxadiazolyl kainic acid 1 (AODKA). AODKA induced a larger calcium influx and a faster processes extension than kainic acid in U118-MG cells. AODKA is a new tool to study KAR activity in the nervous system.Competing Interest StatementThe authors have declared no competing interest.