TY - JOUR T1 - An algebraic solution for determining overall rotational correlation times from cross-correlated relaxation rates JF - bioRxiv DO - 10.1101/2021.01.16.426977 SP - 2021.01.16.426977 AU - Scott A. Robson AU - Joshua J. Ziarek Y1 - 2021/01/01 UR - http://biorxiv.org/content/early/2021/01/19/2021.01.16.426977.abstract N2 - An accurate rotational correlation time is critical for quantitative analysis of fast timescale NMR dynamics. As molecular weights increase, the classic derivation using transverse and longitudinal relaxation rates becomes increasingly unsuitable due to the non-trivial contribution of dipole-dipole and chemical exchange processes. Derivation using cross-correlated relaxation experiments, such as TRACT, overcome these limitations but are erroneously calculated in at approximately 50% of the citing literature. The goals of this study are to 1) investigate the potential sources of the error, 2) provide an algebraic solution, and 3) highlight that future inaccuracies can be minimized by requiring publication of sufficient raw data and computational routes for re-evaluation.Competing Interest StatementThe authors have declared no competing interest. ER -