TY - JOUR T1 - The impact of Spike mutations on SARS-CoV-2 neutralization JF - bioRxiv DO - 10.1101/2021.01.15.426849 SP - 2021.01.15.426849 AU - C Rees-Spear AU - L Muir AU - SA Griffith AU - J Heaney AU - Y Aldon AU - JL Snitselaar AU - P Thomas AU - C Graham AU - J Seow AU - N Lee AU - A Rosa AU - C Roustan AU - CF Houlihan AU - RW Sanders AU - R Gupta AU - P Cherepanov AU - H Stauss AU - E Nastouli AU - on behalf of the SAFER Investigators AU - KJ Doores AU - MJ van Gils AU - LE McCoy Y1 - 2021/01/01 UR - http://biorxiv.org/content/early/2021/01/19/2021.01.15.426849.abstract N2 - Multiple SARS-CoV-2 vaccines have shown protective efficacy, which is most likely mediated by neutralizing antibodies recognizing the viral entry protein, Spike. Antibodies from SARS-CoV-2 infection neutralize the virus by focused targeting of Spike and there is limited serum cross-neutralization of the closely-related SARS-CoV. As new SARS-CoV-2 variants are rapidly emerging, exemplified by the B.1.1.7, 501Y.V2 and P.1 lineages, it is critical to understand if antibody responses induced by infection with the original SARS-CoV-2 virus or the current vaccines will remain effective against virus variants. In this study we evaluate neutralization of a series of mutated Spike pseudotypes including a B.1.1.7 Spike pseudotype. The analyses of a panel of Spike-specific monoclonal antibodies revealed that the neutralizing activity of some antibodies was dramatically reduced by Spike mutations. In contrast, polyclonal antibodies in the serum of patients infected in early 2020 remained active against most mutated Spike pseudotypes. The majority of serum samples were equally able to neutralize the B.1.1.7 Spike pseudotype, however potency was reduced in a small number of samples (3 of 36) by 5–10-fold. This work highlights that changes in the SARS-CoV-2 Spike can alter neutralization sensitivity and underlines the need for effective real-time monitoring of emerging mutations and their impact on vaccine efficacy.Competing Interest StatementThe authors have declared no competing interest. ER -