RT Journal Article
SR Electronic
T1 All-optical electrophysiology in hiPSC-derived neurons with synthetic voltage sensors
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 2021.01.18.427081
DO 10.1101/2021.01.18.427081
A1 Francesca Puppo
A1 Sanaz Sadegh
A1 Cleber A. Trujillo
A1 Martin Thunemann
A1 Evan Campbell
A1 Matthieu Vandenberghe
A1 Xiwei Shan
A1 Ibrahim A Akkouh
A1 Evan W. Miller
A1 Brenda L. Bloodgood
A1 Gabriel A. Silva
A1 Anders M. Dale
A1 Gaute T. Einevoll
A1 Srdjan Djurovic
A1 Ole A. Andreassen
A1 Alysson R. Muotri
A1 Anna Devor
YR 2021
UL http://biorxiv.org/content/early/2021/01/19/2021.01.18.427081.abstract
AB Voltage imaging and “all-optical electrophysiology” in human induced pluripotent stem cell (hiPSC)-derived neurons have opened unprecedented opportunities for high-throughput phenotyping of activity in neurons possessing unique genetic backgrounds of individual patients. While prior all-optical electrophysiology studies relied on genetically encoded voltage indicators, viral transduction of human neurons with large or multiple expression vectors can impact cell function and often lead to massive cell death. Here, we demonstrate an alternative protocol using a synthetic voltage sensor and genetically encoded optogenetic actuator that generate robust and reproducible results. We demonstrate the functionality of this method by measuring spontaneous and evoked activity in three independent hiPSC-derived neuronal cell lines with distinct genetic backgrounds.