TY - JOUR T1 - The pseudouridine synthase dyskerin binds to cytoplasmic H/ACA-box snoRNA retaining transcripts affecting nuclear hormone receptor dependence JF - bioRxiv DO - 10.1101/2021.01.21.427585 SP - 2021.01.21.427585 AU - Federico Zacchini AU - Giulia Venturi AU - Veronica De Sanctis AU - Roberto Bertorelli AU - Claudio Ceccarelli AU - Donatella Santini AU - Mario Taffurelli AU - Marianna Penzo AU - Davide Treré AU - Alberto Inga AU - Erik Dassi AU - Lorenzo Montanaro Y1 - 2021/01/01 UR - http://biorxiv.org/content/early/2021/01/21/2021.01.21.427585.abstract N2 - Dyskerin is a nuclear protein involved in H/ACA box snoRNA-guided uridine modification of RNA. Since its defective function induces specific alterations in gene expression, we sought to unbiasedly identify mRNAs regulated by dyskerin. We found that dyskerin depletion affects the expression or the association with polysomes of selected mRNA isoforms characterized by the retention of H/ACA box snoRNA-containing introns. These snoRNA retaining transcripts (snoRTs) are bound by dyskerin and can interact with cytoplasmic ribosomes. We then characterized the cytoplasmic dyskerin RNA interactome finding both H/ACA box snoRTs and protein-coding transcripts. Since a fraction of these latter transcripts is involved in the nuclear hormone receptor binding, we tested to see if this specific activity is affected by dyskerin. Results indicate that dyskerin dysregulation may alter the dependence on nuclear hormone receptor ligands in breast cancer. Our work suggests a cytoplasmic function for dyskerin which could affect mRNA post-transcriptional networks relevant for nuclear hormone receptor functions.Competing Interest StatementThe authors have declared no competing interest. ER -