TY - JOUR T1 - Human neuronal networks on micro-electrode arrays are a highly robust tool to study disease-specific genotype-phenotype correlations <em>in vitro</em> JF - bioRxiv DO - 10.1101/2021.01.20.427439 SP - 2021.01.20.427439 AU - B. Mossink AU - A.H.A. Verboven AU - E.J.H. van Hugte AU - T.M. Klein Gunnewiek AU - G. Parodi AU - K. Linda AU - C. Schoenmaker AU - T. Kleefstra AU - T. Kozicz AU - H. van Bokhoven AU - D. Schubert AU - N. Nadif Kasri AU - M. Frega Y1 - 2021/01/01 UR - http://biorxiv.org/content/early/2021/01/21/2021.01.20.427439.abstract N2 - Micro-electrode arrays (MEAs) are increasingly used to characterize neuronal network activity of human induced pluripotent stem-cell (hiPSC)-derived neurons. Despite their gain in popularity, MEA recordings from hiPSC-derived neuronal networks are not always used to their full potential in respect to experimental design, execution and data analysis. Therefore, we benchmarked the robustness and sensitivity of MEA-derived neuronal activity patterns derived from ten healthy individual control lines. We provide recommendations on experimental design and analysis to achieve standardization. With such standardization, MEAs can be used as a reliable platform to distinguish (disease-specific) network phenotypes. In conclusion, we show that MEAs are a powerful and robust tool to uncover functional neuronal network phenotypes from hiPSC-derived neuronal networks, and provide an important resource to advance the hiPSC field towards the use of MEAs for disease-phenotyping and drug discovery.Competing Interest StatementThe authors have declared no competing interest. ER -