PT  - JOURNAL ARTICLE
AU  - Adam K. Glaser
AU  - Nicholas P. Reder
AU  - Ye Chen
AU  - Chengbo Yin
AU  - Linpeng Wei
AU  - Soyoung Kang
AU  - Lindsey A. Barner
AU  - Weisi Xie
AU  - Erin F. McCarty
AU  - Chenyi Mao
AU  - Aaron R. Halpern
AU  - Caleb R. Stoltzfus
AU  - Jonathan S. Daniels
AU  - Michael Y. Gerner
AU  - Philip R. Nicovich
AU  - Joshua C. Vaughan
AU  - Lawrence D. True
AU  - Jonathan T.C. Liu
TI  - Multi-immersion open-top light-sheet microscope for high-throughput imaging of cleared tissues
AID  - 10.1101/548107
DP  - 2019 Jan 01
TA  - bioRxiv
PG  - 548107
4099  - http://biorxiv.org/content/early/2019/02/13/548107.short
4100  - http://biorxiv.org/content/early/2019/02/13/548107.full
AB  - Recent advances in optical clearing and light-sheet microscopy have provided unprecedented access to structural and molecular information from intact tissues. However, current light-sheet microscopes have imposed constraints on the size, shape, number of specimens, and compatibility with various clearing protocols. Here we present a multi-immersion open-top light-sheet microscope that enables simple mounting of multiple specimens processed with a variety of protocols, which will facilitate wider adoption by preclinical researchers and clinical laboratories.One Sentence Summary Glaser et al. describe a multi-immersion open-top light-sheet microscope that enables simple and high-throughput imaging of large numbers of preclinical and clinical specimens prepared with a variety of clearing protocols.