RT Journal Article
SR Electronic
T1 Optimized antibody humanization by intra and inter VH-VL binding energy sorting
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 2021.01.06.425645
DO 10.1101/2021.01.06.425645
A1 Qiang Zhong
YR 2021
UL http://biorxiv.org/content/early/2021/01/25/2021.01.06.425645.abstract
AB Humanization of non-human derived antibody is necessary to reduce the immunogenicity of antibody drug. In recent years, computer aided antibody humanization became an efficient and rapid routine process. We report here a new computational humanization pipeline which includes CDR grafting onto the crystal structures of homologous antibody and CDR grafted humanized antibody structures. Then, the intra and inter VH-VL binding energies are calculated and sorted for optimized humanized antibody. The resulted humanized antibodies are ranked and compared to experimental dataset, which confirms the validity of humanized antibody design by intra and inter VH-VL binding energy.Competing Interest StatementThe program and pipeline of antibody design are covered in pending patents. Employees of IBP have stock holdings in IBP.