RT Journal Article
SR Electronic
T1 Genome-wide identification and analysis of A-to-I RNA editing events in the malignantly transformed cell lines from BEP2D induced by α-particles radiation
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 551499
DO 10.1101/551499
A1 Qiaowei Liu
A1 Hao Li
A1 Lukuan You
A1 Tao Li
A1 Lingling Li
A1 Pingkun Zhou
A1 Xiaochen Bo
A1 Hebing Chen
A1 Xiaohua Chen
A1 Yi Hu
YR 2019
UL http://biorxiv.org/content/early/2019/02/15/551499.abstract
AB Adenosine (A) to inosine (I) RNA editing is the most prevalent RNA editing mechanism in humans and play critical roles in tumorigenesis. However, the effects of radiation on RNA editing and the mechanisms of radiation-induced cancer were poorly understood. Here, we analyzed human bronchial epithelial BEP2D cells and radiation-induced malignantly transformed cells with next generation sequencing. By performing an integrated analysis of A-to-I RNA editing, we found that genome-encoded single-nucleotide polymorphisms (SNPs) might induce the downregulation of ADAR2 enzymes, and further caused the abnormal occurrence of RNA editing in malignantly transformed cells. These editing events were significantly enriched in differentially expressed genes between normal cells and cancer cells. In addition, oncogenes CTNNB1 and FN1 were highly edited and significantly overexpressed in cancer cells, thus may be responsible for the lung cancer progression. Our work provides a systematic analysis of RNA editing from lung tumor specimens with high-throughput RNA sequencing and DNA sequencing. Moreover, these results demonstrate further evidence for RNA editing as an important tumorigenesis mechanism.