PT - JOURNAL ARTICLE AU - Taiki Mori AU - Toru Hashimoto AU - Yoshimi Sakai TI - Evaluating the tea bag method as a potential tool for detecting the effects of added nutrients and their interactions with climate on litter decomposition AID - 10.1101/2021.01.28.428520 DP - 2021 Jan 01 TA - bioRxiv PG - 2021.01.28.428520 4099 - http://biorxiv.org/content/early/2021/01/30/2021.01.28.428520.short 4100 - http://biorxiv.org/content/early/2021/01/30/2021.01.28.428520.full AB - It is acknowledged that exogenous nutrient addition often stimulates early-stage litter decomposition in forests and late-stage decomposition is generally suppressed by nitrogen addition, whereas the interactive effects of nutrient addition and abiotic environmental factors, such as climate, on decomposition remain unclear. The tea bag method, which was developed to provide the decomposition rate constant k of early-stage decomposition and stabilization factor S of labile materials in the late stage, is a potentially useful tool for examining the impacts of nutrient addition on both early- and late-stage litter decomposition and their interactions with climate. At a long-term (38-year) continuous fertilization experimental site (an Abies sachalinensis Fr. Schmidt stand) in Hokkaido, Japan, we examined whether a standard tea bag method protocol was sufficiently sensitive to reveal any impacts of nutrient addition on early- and late-stage decomposition. In addition, we tested the interactive effects of nutrient addition and climate on litter decomposition. The short incubation period of the tea bag method (ca. 90 days) enabled us to obtain decomposition data from the same location at three different times in a year, i.e., early summer, midsummer, and winter, providing an opportunity to test interactive effects. We demonstrated that the decomposition rate of rooibos tea and the decomposition rate constant k of early-stage decomposition were clearly stimulated by fertilization in midsummer, but no impacts were detected in other seasons, probably because the relative importance of nutrient availability was elevated in midsummer, during which decomposition rates were less constrained by temperature and moisture. The green tea decomposition rate and stabilization factor S, an index related to late-stage decomposition, were unaffected by fertilization. This was probably because the tea bag method does not take into account lignin degradation, which is considered a key factor controlling late-stage litter decomposition. Overall, the present study (i) successfully determined the interactive effects of nutrient addition and climate factors on litter decomposition by making full use of the tea bag method, and (ii) the results suggest that the tea bag method can be a suitable tool for examining the direct effects of nutrient addition and their interactions with environmental factors on early-stage litter decomposition, but not those on late-stage decomposition.Competing Interest StatementThe authors have declared no competing interest.