PT  - JOURNAL ARTICLE
AU  - Maria Fasolino
AU  - Gregory W. Schwartz
AU  - Maria L. Golson
AU  - Yue J. Wang
AU  - Ashleigh Morgan
AU  - Chengyang Liu
AU  - Jonathan Schug
AU  - Jinping Liu
AU  - Minghui Wu
AU  - Daniel Traum
AU  - Ayano Kondo
AU  - Catherine L. May
AU  - Naomi Goldman
AU  - Wenliang Wang
AU  - the HPAP Consortium
AU  - Michael Feldman
AU  - Jason H. Moore
AU  - Alberto S. Japp
AU  - Michael R. Betts
AU  - Robert B. Faryabi
AU  - Ali Naji
AU  - Klaus H. Kaestner
AU  - Golnaz Vahedi
TI  - Multiomics single-cell analysis of human pancreatic islets reveals novel cellular states in health and type 1 diabetes
AID  - 10.1101/2021.01.28.428598
DP  - 2021 Jan 01
TA  - bioRxiv
PG  - 2021.01.28.428598
4099  - http://biorxiv.org/content/early/2021/01/31/2021.01.28.428598.short
4100  - http://biorxiv.org/content/early/2021/01/31/2021.01.28.428598.full
AB  - Type 1 diabetes (T1D) is an autoimmune disease of only partially defined etiology in which immune cells destroy insulin-producing beta cells. Using single-cell transcriptomics and an advanced analytical strategy to assess pancreatic islets of T1D, autoantibody-positive, and non-diabetic organ donors, we identified both canonical cell types and rare insulin-expressing cells with a hybrid mixture of endocrine and exocrine gene signatures within all donors. We further found elevated expression of MHC Class II pathway genes in exocrine ductal cells of T1D donors, which we confirmed through CyTOF, in situ imaging mass cytometry, and immunofluorescence analysis. Taken together, our multimodal analyses identify novel cell types and processes that may contribute to T1D immunopathogenesis and provide new cellular and molecular insights into human pancreas function.Competing Interest StatementThe authors have declared no competing interest.