TY - JOUR T1 - <em>Drosophila</em> Evolution over Space and Time (DEST) - A New Population Genomics Resource JF - bioRxiv DO - 10.1101/2021.02.01.428994 SP - 2021.02.01.428994 AU - Martin Kapun AU - Joaquin C. B. Nunez AU - María Bogaerts-Márquez AU - Jesús Murga-Moreno AU - Margot Paris AU - Joseph Outten AU - Marta Coronado-Zamora AU - Courtney Tern AU - Omar Rota-Stabelli AU - Maria P. García Guerreiro AU - Sònia Casillas AU - Dorcas J. Orengo AU - Eva Puerma AU - Maaria Kankare AU - Lino Ometto AU - Volker Loeschcke AU - Banu S. Onder AU - Jessica K. Abbott AU - Stephen W. Schaeffer AU - Subhash Rajpurohit AU - Emily L Behrman AU - Mads F. Schou AU - Thomas J.S. Merritt AU - Brian P Lazzaro AU - Amanda Glaser-Schmitt AU - Eliza Argyridou AU - Fabian Staubach AU - Yun Wang AU - Eran Tauber AU - Svitlana V. Serga AU - Daniel K. Fabian AU - Kelly A. Dyer AU - Christopher W. Wheat AU - John Parsch AU - Sonja Grath AU - Marija Savic Veselinovic AU - Marina Stamenkovic-Radak AU - Mihailo Jelic AU - Antonio J. Buendía-Ruíz AU - M. Josefa Gómez-Julián AU - M. Luisa Espinosa-Jimenez AU - Francisco D. Gallardo-Jiménez AU - Aleksandra Patenkovic AU - Katarina Eric AU - Marija Tanaskovic AU - Anna Ullastres AU - Lain Guio AU - Miriam Merenciano AU - Sara Guirao-Rico AU - Vivien Horváth AU - Darren J. Obbard AU - Elena Pasyukova AU - Vladimir E. Alatortsev AU - Cristina P. Vieira AU - Jorge Vieira AU - J. Roberto Torres AU - Iryna Kozeretska AU - Oleksandr M. Maistrenko AU - Catherine Montchamp-Moreau AU - Dmitry V. Mukha AU - Heather E. Machado AU - Antonio Barbadilla AU - Dmitri Petrov AU - Paul Schmidt AU - Josefa Gonzalez AU - Thomas Flatt AU - Alan O. Bergland Y1 - 2021/01/01 UR - http://biorxiv.org/content/early/2021/02/01/2021.02.01.428994.abstract N2 - Drosophila melanogaster is a premier model in population genetics and genomics, and a growing number of whole-genome datasets from natural populations of this species have been published over the last 20 years. A major challenge is the integration of these disparate datasets, often generated using different sequencing technologies and bioinformatic pipelines, which hampers our ability to address questions about the evolution and population structure of this species. Here we address these issues by developing a bioinformatics pipeline that maps pooled sequencing (Pool-Seq) reads from D. melanogaster to a hologenome consisting of fly and symbiont genomes and estimates allele frequencies using either a heuristic (PoolSNP) or a probabilistic variant caller (SNAPE-pooled). We use this pipeline to generate the largest data repository of genomic data available for D. melanogaster to date, encompassing 271 population samples from over 100 locations in &gt;20 countries on four continents. Several of these locations are sampled at different seasons across multiple years. This dataset, which we call Drosophila Evolution over Space and Time (DEST), is coupled with sampling and environmental meta-data. A web-based genome browser and web portal provide easy access to the SNP dataset. Our aim is to provide this scalable platform as a community resource which can be easily extended via future efforts for an even more extensive cosmopolitan dataset. Our resource will enable population geneticists to analyze spatio-temporal genetic patterns and evolutionary dynamics of D. melanogaster populations in unprecedented detail.Competing Interest StatementThe authors have declared no competing interest. ER -