RT Journal Article
SR Electronic
T1 Assessment of single cell RNA-seq normalization methods
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 064329
DO 10.1101/064329
A1 Bo Ding
A1 Lina Zheng
A1 Wei Wang
YR 2016
UL http://biorxiv.org/content/early/2016/07/17/064329.abstract
AB We have assessed the performance of seven normalization methods for single cell RNA-seq using data generated from dilution of RNA samples. Our analyses showed that methods considering spike-in ERCC RNA molecules significantly outperformed those not considering ERCCs. This work provides a guidance of selecting normalization methods to remove technical noise in single cell RNA-seq data.