TY - JOUR T1 - Intermolecular interactions drive protein adaptive and co-adaptive evolution at both species and population levels JF - bioRxiv DO - 10.1101/2021.02.08.430345 SP - 2021.02.08.430345 AU - Junhui Peng AU - Li Zhao Y1 - 2021/01/01 UR - http://biorxiv.org/content/early/2021/02/10/2021.02.08.430345.abstract N2 - Proteins are the building blocks for almost all the functions in cells. Understanding the molecular evolution of proteins and the forces that shape protein evolution is an essential step in understanding the basis of function and evolution. Previous studies have shown that adaptation occurs frequently at the protein surface, such as in genes involved in host-pathogen interactions. However, it remains unclear whether adaptive sites are distributed randomly or at regions that are associated with particular structural or functional characteristics across the genome, since many of the proteins lack structural or functional annotations. Here, we seek to tackle this question by combining large-scale bioinformatic prediction, structural analysis, phylogenetic inference, and population genomic analysis of Drosophila protein-coding genes. By estimating and comparing the rate of adaptive substitutions at protein and residue level, we showed that adaptation is more relevant to function-related rather than structure-related properties. Among the function-related properties, we found that molecular interactions in proteins contribute to adaptive evolution, and putative binding residues exhibit higher rates of adaptation. We observed that physical interactions might play a role in the co-adaptation of fast-adaptive proteins. We found that strongly differentiated amino acids in protein coding genes are mostly adaptive, which may contribute to the long-term adaptive evolution. Our results suggest important roles of intermolecular interactions and co-adaptation in the adaptive evolution of proteins both at the species and population levels.Competing Interest StatementThe authors have declared no competing interest. ER -