RT Journal Article
SR Electronic
T1 A system for continuous and automated measurement of mouse home-cage drinking with automated control of liquid access
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 2021.02.10.430693
DO 10.1101/2021.02.10.430693
A1 Bagley, Jared R.
A1 Kashinsky, Wayne M.
A1 Jentsch, James D.
YR 2021
UL http://biorxiv.org/content/early/2021/02/11/2021.02.10.430693.abstract
AB Measurement of drinking behavior in laboratory animals is an often utilized method in many areas of scientific research, including the study of ingestive behaviors and addictions. We have designed a system that measures drinking by continuously tracking fluid-filled bottle weights with load cells and calculating change in fluid weight per drinking bout. The load cells serve both as a contact sensor that detects mouse-spout contact, as well as a monitor of fluid weight change per contact bout. The design described here fulfills several key criteria, including automated and continuous recording of drinking in the home-cage, automated control of liquid access, and inexpensive/reproducible fabrication. These features may allow researchers to generate high-resolution, detailed information on drinking behavior in high-throughput experimental designs. Here, we provide an overview of the design and present results from tests to validate the system. C57BL/6J mice were offered water and ethanol concurrently, using this system. Consumption weights were determined by the system and independently by conventional approaches. The results indicated a near-perfect correlation between the two methods, indicating the system returned valid consumption weights. This system functions as a valid drinking monitor that provides temporally precise data with a low cost design.Competing Interest StatementThe authors have declared no competing interest.