RT Journal Article SR Electronic T1 An improved ChEC-seq method accurately maps the genome-wide binding of transcription coactivators and sequence-specific transcription factors JF bioRxiv FD Cold Spring Harbor Laboratory SP 2021.02.12.430999 DO 10.1101/2021.02.12.430999 A1 Rafal Donczew A1 Amélia Lalou A1 Didier Devys A1 Laszlo Tora A1 Steven Hahn YR 2021 UL http://biorxiv.org/content/early/2021/02/12/2021.02.12.430999.abstract AB Mittal and colleagues have raised questions about mapping transcription factor locations on DNA using the MNase-based ChEC-seq method (Mittal et al., 2021). Partly due to this concern, we modified the experimental conditions of the MNase cleavage step and subsequent computational analyses, resulting in more stringent conditions for mapping protein-DNA interactions (Donczew et al., 2020). The revised method (dx.doi.org/10.17504/protocols.io.bizgkf3w) answers questions raised by Mittal et al. and, without changing earlier conclusions, identified widespread promoter binding of the transcription coactivators TFIID and SAGA at active genes. The revised method is also suitable for accurately mapping the genome-wide locations of DNA sequence-specific transcription factors.Competing Interest StatementThe authors have declared no competing interest.