RT Journal Article
SR Electronic
T1 Sterile Inflammation Alters Neutrophil Kinetics in Mice
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 2021.02.12.430891
DO 10.1101/2021.02.12.430891
A1 Alakesh Singh
A1 Thiruvickraman Jothiprakasam
A1 Jayashree V. Raghavan
A1 Siddharth Jhunjhunwala
YR 2021
UL http://biorxiv.org/content/early/2021/02/13/2021.02.12.430891.abstract
AB Neutrophils play a crucial role in establishing inflammation in response to an infection or injury, but their production rates, as well as blood and tissue residence times, remain poorly characterized under these conditions. Herein, using a biomaterial implant model to establish inflammation followed by in vivo tracking of newly formed neutrophils, we determine neutrophil kinetics under inflammatory conditions. To obtain quantifiable information from our experimental observations, we develop an ordinary differential equation-based mathematical model to extract kinetic parameters. Our data show that in the presence of inflammation resulting in emergency granulopoiesis-like conditions, neutrophil maturation time in the bone marrow and half-life in the blood reduces by about 40%, compared to non-inflammatory conditions. Additionally, neutrophil residence time at the inflammatory site increases by two-fold. Together, these data improve our understanding of neutrophil kinetics under inflammatory conditions, which could pave the way for therapies that focus on modulating in vivo neutrophil dynamics.Competing Interest StatementThe authors have declared no competing interest.