RT Journal Article
SR Electronic
T1 Whole plastid genome-based phylogenomics supports an inner placement of the <em>O. insectifera</em> group rather than a basal position in the rapidly diversifying <em>Ophrys</em> genus (Orchidaceae)
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 2020.12.16.423003
DO 10.1101/2020.12.16.423003
A1 Bertrand, Joris A. M.
A1 Gibert, Anaïs
A1 Llauro, Christel
A1 Panaud, Olivier
YR 2021
UL http://biorxiv.org/content/early/2021/02/16/2020.12.16.423003.abstract
AB Some lineages of the Orchid genus Ophrys exhibit among the highest diversification rates reported so far. As a consequence of a such intense and rapid evolution, the systematics and the taxonomy of this genus remains unclear. A hybrid assembly approach based-on long- and short-read genomic data allowed us to outperform classical methods to successfully assemble whole plastid genomes for two new Ophrys species: O. aymoninii and O. lutea. Along with three other previously Ophrys plastid genome sequences, we then reconstructed the first whole plastome-based molecular phylogeny including representatives of the three mains recognized Ophrys lineages. Our results support the placement of the O. insectifera clade as sister group of ‘non-basal Ophrys’ rather than a basal position. Our findings corroborate recent results obtained from genomic data (RAD-seq and transcriptomes) but contrast with previous ones. These results therefore confirm that molecular phylogenetic hypotheses based on a limited number of loci (e.g. nrITS, matK, rbcL) may have provided a biased picture of phylogenetic relationships within Ophrys and possibly other plant taxa.Competing Interest StatementThe authors have declared no competing interest.