PT  - JOURNAL ARTICLE
AU  - Bakas, Spyridon
AU  - Uttamchandani, Deepak
AU  - Toshiyoshi, Hiroshi
AU  - Bauer, Ralf
TI  - MEMS enabled miniaturized light-sheet microscopy with all optical control
AID  - 10.1101/2021.02.13.431066
DP  - 2021 Jan 01
TA  - bioRxiv
PG  - 2021.02.13.431066
4099  - http://biorxiv.org/content/early/2021/02/16/2021.02.13.431066.short
4100  - http://biorxiv.org/content/early/2021/02/16/2021.02.13.431066.full
AB  - We have designed and implemented a compact, cost-efficient miniaturised light-sheet microscopy system based on optical Microelectromechanical Systems (MEMS) scanners and tunable lenses. The system occupies a footprint of 20 × 28 × 13 cm3 and combines off-the-shelf optics and optomechanics with 3D-printed structural and optical elements, and an economically costed objective lens, excitation laser and camera. All-optical volume scanning enables imaging of 340 × 190 × 60 µm3 volumes with 0.25 vps and minimum lateral and axial resolution of 0.9 µm and 2.95 µm respectively. An open-top geometry allows imaging of samples on flat bottomed holders, allowing integration with microfluidic devices, multi-well plates and slide mounted samples, with applications envisaged in biomedical research and pre-clinical settings.Competing Interest StatementThe authors have declared no competing interest.