TY - JOUR T1 - Automated Feature Extraction from Large Cardiac Electrophysiological Data Sets JF - bioRxiv DO - 10.1101/2020.10.21.340968 SP - 2020.10.21.340968 AU - John Jurkiewicz AU - Stacie Kroboth AU - Viviana Zlochiver AU - Peter Hinow Y1 - 2021/01/01 UR - http://biorxiv.org/content/early/2021/02/16/2020.10.21.340968.abstract N2 - Rationale A new multi-electrode array-based application for the long-term recording of action potentials from electrogenic cells makes possible exciting cardiac electrophysiology studies in health and disease. With hundreds of simultaneous electrode recordings being acquired over a period of days, the main challenge becomes achieving reliable signal identification and quantification.Objective We set out to develop an algorithm capable of automatically extracting regions of high-quality action potentials from terabyte size experimental results and to map the trains of action potentials into a low-dimensional feature space for analysis.Methods and Results Our automatic segmentation algorithm finds regions of acceptable action potentials in large data sets of electrophysiological readings. We use spectral methods and support vector machines to classify our readings and to extract relevant features. We are able to show that action potentials from the same cell site can be recorded over days without detrimental effects to the cell membrane. The variability between measurements 24 h apart is comparable to the natural variability of the features at a single time point.Conclusions Our work contributes towards a non-invasive approach for cardiomyocyte functional maturation, as well as developmental, pathological and pharmacological studies. As the human-derived cardiac model tissue has the genetic makeup of its donor, a powerful tool for individual drug toxicity screening emerges.Competing Interest StatementThe authors have declared no competing interest.APaction potentialFPfield potentialMEAmulti-electrode arrayhiPSC-CMshuman induced pluripotent stem cell derived cardiomyocytesAPD/APDmaction potential duration (to m % level)BCLbasic cycle lengthDIdiastolic intervalDFTdiscrete Fourier transformSVMsupport vector machine ER -