PT - JOURNAL ARTICLE AU - Karla J. Suchacki AU - Carlos J. Alcaide-Corral AU - Samah Nimale AU - Mark G. Macaskill AU - Roland H. Stimson AU - Colin Farquharson AU - Tom C. Freeman AU - Adriana A. S. Tavares TI - A systems-level analysis of dynamic total-body PET data reveals complex skeletal energy metabolism networks <em>in vivo</em> AID - 10.1101/2021.02.16.431368 DP - 2021 Jan 01 TA - bioRxiv PG - 2021.02.16.431368 4099 - http://biorxiv.org/content/early/2021/02/17/2021.02.16.431368.short 4100 - http://biorxiv.org/content/early/2021/02/17/2021.02.16.431368.full AB - Bone is now regarded to be a key regulator of a number of metabolic processes, in addition to the regulation of mineral metabolism. However, our understanding of complex bone metabolic interactions at a systems level remains rudimentary, limiting our ability to assess systemic mechanisms underlying diseases and develop novel therapeutics. In vitro molecular biology and bioinformatics approaches have frequently been used to understand the mechanistic changes underlying disease at the cell level, however, these approaches lack the capability to interrogate dynamic multi-bone metabolic interactions in vivo. Here we present a novel and integrative approach to understand complex bone metabolic interactions in vivo using total-body positron emission tomography (PET) network analysis of murine 18F-FDG scans, as a biomarker of glucose metabolism signature in bones. In this report we show that different bones within the skeleton have a unique glucose metabolism and form a complex metabolic network. These data could have important therapeutic implications in the management of the metabolic syndrome and skeletal disease. The application of our approach to clinical and preclinical total-body PET studies promises to reveal further physiological and pathological tissue interactions, which simplistic PET standard uptake values analysis fail to interrogate, extending beyond skeletal metabolism, due to the diversity of PET radiotracers available and under development as well as the advent of clinical total-body PET systems.One Sentence Summary Bones form a complex metabolic network.Competing Interest StatementThe authors have declared no competing interest.