%0 Journal Article %A Gytis Dudas %A Greg Huber %A Michael Wilkinson %A David Yllanes %T Polymorphism of Genetic Ambigrams %D 2021 %R 10.1101/2021.02.16.431493 %J bioRxiv %P 2021.02.16.431493 %X Double synonyms in the genetic code can be used as a tool to test competing hypotheses regarding ambigrammatic narnavirus genomes. Applying the analysis to recent observations of Culex narnavirus 1 and Zhejiang mosquito virus 3 ambigrammatic viruses indicates that the open reading frame on the complementary strand of the segment coding for RNA-dependent RNA polymerase does not code for a functional protein. Culex narnavirus 1 has been shown to possess a second segment, also ambigrammatic, termed ‘Robin’. We find a comparable segment for Zhejiang mosquito virus 3, a moderately diverged relative of Culex narnavirus 1. Our analysis of Robin polymorphisms suggests that its reverse open reading frame also does not code for a protein. We make a hypothesis about its role.Competing Interest StatementThe authors have declared no competing interest. %U https://www.biorxiv.org/content/biorxiv/early/2021/02/17/2021.02.16.431493.full.pdf