PT  - JOURNAL ARTICLE
AU  - Thomas J. Kucharski
AU  - Rufus Hards
AU  - Kristina M. Godek
AU  - Scott A. Gerber
AU  - Duane A. Compton
TI  - Chromosome segregation fidelity is controlled by small changes in phospho-occupancy at the kinetochore-microtubule interface
AID  - 10.1101/2021.02.16.431549
DP  - 2021 Jan 01
TA  - bioRxiv
PG  - 2021.02.16.431549
4099  - http://biorxiv.org/content/early/2021/02/17/2021.02.16.431549.short
4100  - http://biorxiv.org/content/early/2021/02/17/2021.02.16.431549.full
AB  - Kinetochore protein phosphorylation promotes the correction of erroneous microtubule attachments to ensure faithful chromosome segregation during cell division. Determining how phosphorylation executes error correction requires an understanding of whether kinetochore substrates are completely (i.e. all-or-none) or only fractionally phosphorylated. Using quantitative mass spectrometry (MS), we measured phospho-occupancy on the conserved kinetochore protein Hec1 (NDC80) that directly binds microtubules. None of the positions measured exceeded ∼50% phospho-occupancy, and the cumulative phospho-occupancy changed by only ∼20% in response to changes in microtubule attachment status. The narrow dynamic range of phospho-occupancy is maintained by ongoing phosphatase activity. Further, both Cdk1-Cyclin B1 and Aurora kinases phosphorylate Hec1 to enhance error correction in response to different types of microtubule attachment errors. Thus, networks of kinases and phosphatases maintain low inherent phospho-occupancy to promote microtubule attachment to kinetochores while providing for high sensitivity of kinetochore-microtubule attachments to very small changes in phospho-occupancy to ensure high mitotic fidelity.