RT Journal Article
SR Electronic
T1 Localization microscopy at doubled precision with patterned illumination
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 554337
DO 10.1101/554337
A1 Jelmer Cnossen
A1 Taylor Hinsdale
A1 Rasmus Ø. Thorsen
A1 Florian Schueder
A1 Ralf Jungmann
A1 Carlas S. Smith
A1 Bernd Rieger
A1 Sjoerd Stallinga
YR 2019
UL http://biorxiv.org/content/early/2019/02/20/554337.abstract
AB MINFLUX offers a breakthrough in single molecule localization precision, but suffers from a tiny field-of-view and a lack of practical parallelism. Here, we combine centroid estimation and illumination pattern induced photon count variations in a conventional widefield imaging setup to extract position information over a typical micron sized field-of-view. We show a near twofold improvement in precision over standard localization with the same photon count on DNA-origami nano-structures.