RT Journal Article
SR Electronic
T1 Long SARS-CoV-2 nucleocapsid sequences in blood monocytes collected soon after hospital admission
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 2020.12.16.423113
DO 10.1101/2020.12.16.423113
A1 Nathan Pagano
A1 Maudry Laurent-Rolle
A1 Jack Chun-Chieh Hsu
A1 the Yale IMPACT Research Team
A1 Chantal BF Vogels
A1 Nathan D Grubaugh
A1 Laura Manuelidis
YR 2021
UL http://biorxiv.org/content/early/2021/02/19/2020.12.16.423113.abstract
AB Many viruses infect circulating mononuclear cells thereby facilitating infection of diverse organs. Blood monocytes (PBMC) are being intensively studied as immunologic and pathologic responders to the new SARS-CoV-2 virus (CoV19) but direct evidence showing CoV19 in monocytes is lacking. Circulating myeloid cells that take up residence in various organs can harbor viral genomes for many years in lymphatic tissues and brain, and act as a source for re-infection and/or post-viral organ pathology. Because nucleocapsid (NC) proteins protect the viral genome we tested PBMC from acutely ill patients for the diagnostic 72bp NC RNA plus adjacent longer (301bp) transcripts. In 2/11 patient PBMC, but no uninfected controls, long NCs were positive as early as 2-6 days after hospital admission as validated by sequencing. Pathogenic viral fragments, or the infectious virus, are probably disseminated by rare myeloid migratory cells that incorporate CoV19 by several pathways. Predictably, these cells carried CoV19 to heart and brain educing the late post-viral pathologies now evident.Competing Interest StatementThe authors have declared no competing interest.