RT Journal Article
SR Electronic
T1 Establishment of a human three-dimensional chip-based chondro-synovial co-culture joint model for reciprocal cross-talk studies in arthritis research
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 2021.02.19.431936
DO 10.1101/2021.02.19.431936
A1 Mario Rothbauer
A1 Ruth A. Byrne
A1 Silvia Schobesberger
A1 Isabel Olmos Calvo
A1 Anita Fischer
A1 Eva I. Reihs
A1 Sarah Spitz
A1 Barbara Bachmann
A1 Florian Sevelda
A1 Johannes Holinka
A1 Wolfgang Holnthoner
A1 Heinz Redl
A1 Stefan Tögel
A1 Reinhard Windhager
A1 Hans P. Kiener
A1 Peter Ertl
YR 2021
UL http://biorxiv.org/content/early/2021/02/19/2021.02.19.431936.abstract
AB Rheumatoid arthritis is characterised by a progressive, intermittent inflammation at the synovial membrane, which ultimately leads to the destruction of the synovial joint. The synovial membrane, which is the joint capsule’s inner layer, is lined with fibroblast-like synoviocytes that are the key player supporting persistent arthritis leading to bone erosion and cartilage destruction. While microfluidic models that model molecular aspects of bone erosion between bone-derived cells and synoviocytes have been established, RA’s synovial-chondral axis has yet not been realised using a microfluidic 3D model based on human patient in vitro cultures. Consequently, we established a chip-based three-dimensional tissue co-culture model that simulates the reciprocal cross-talk between individual synovial and chondral organoids. We now demonstrate that chondral organoids, when co-cultivated with synovial organoids, induce a higher degree of cartilage physiology and architecture and show differential cytokine response compared to their respective monocultures highlighting the importance of reciprocal tissue-level cross-talk in the modelling of arthritic diseases.Competing Interest StatementThe authors have declared no competing interest.