RT Journal Article
SR Electronic
T1 Massively multiplex single-cell Hi-C
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 065052
DO 10.1101/065052
A1 Ramani, Vijay
A1 Deng, Xinxian
A1 Gunderson, Kevin L
A1 Steemers, Frank J
A1 Disteche, Christine M
A1 Noble, William S
A1 Duan, Zhijun
A1 Shendure, Jay
YR 2016
UL http://biorxiv.org/content/early/2016/07/23/065052.abstract
AB We present combinatorial single cell Hi-C, a novel method that leverages combinatorial cellular indexing to measure chromosome conformation in large numbers of single cells. In this proof-of-concept, we generate and sequence combinatorial single cell Hi-C libraries for two mouse and four human cell types, comprising a total of 9,316 single cells across 5 experiments. We demonstrate the utility of single-cell Hi-C data in separating different cell types, identify previously uncharacterized cell-to-cell heterogeneity in the conformational properties of mammalian chromosomes, and demonstrate that combinatorial indexing is a generalizable molecular strategy for single-cell genomics.