TY - JOUR T1 - High Light and High Temperature Reduce Photosynthesis via Different Mechanisms in the C<sub>4</sub> Model <em>Setaria viridis</em> JF - bioRxiv DO - 10.1101/2021.02.20.431694 SP - 2021.02.20.431694 AU - Cheyenne M. Anderson AU - Erin M. Mattoon AU - Ningning Zhang AU - Eric Becker AU - William McHargue AU - Jiani Yang AU - Dhruv Patel AU - Oliver Dautermann AU - Scott A. M. McAdam AU - Tonantzin Tarin AU - Sunita Pathak AU - Tom J. Avenson AU - Jeffrey Berry AU - Maxwell Braud AU - Krishna K. Niyogi AU - Margaret Wilson AU - Dmitri A. Nusinow AU - Rodrigo Vargas AU - Kirk J. Czymmek AU - Andrea L. Eveland AU - Ru Zhang Y1 - 2021/01/01 UR - http://biorxiv.org/content/early/2021/02/20/2021.02.20.431694.abstract N2 - C4 plants frequently experience damaging high light (HL) and high temperature (HT) conditions in native environments, which reduce growth and yield. However, the mechanisms underlying these stress responses in C4 plants have been under-explored, especially the coordination between mesophyll (M) and bundle sheath (BS) cells. We investigated how the C4 model plant Setaria viridis responded to a four-hour HL or HT treatment at the photosynthetic, transcriptomic, and ultrastructural levels. Although we observed a comparable reduction of photosynthetic efficiency in HL- or HT-treated leaves, detailed analysis of multi-level responses revealed important differences in key pathways and M/BS specificity responding to HL and HT. We provide a systematic analysis of HL and HT responses in S. viridis, reveal different acclimation strategies to these two stresses in C4 plants, discover unique light/temperature responses in C4 plants in comparison to C3 plants, and identify potential targets to improve abiotic stress tolerance in C4 crops.Competing Interest StatementThe authors have declared no competing interest.HLhigh lightHThigh temperaturectrlcontrolMmesophyllBSbundle sheathRubiscoribulose-1,5-bisphosphate carboxylase/oxygenasePSIIphotosystem IIPSIphotosystem IANetnet CO2 assimilation ratesDEGsdifferentially expressed genesNPQnon-photochemical quenchingCEFcyclic electron flow around PSIRCARubisco activaseABAabscisic acidPGplastoglobuliHSPheat shock proteinHSFheat shock transcription factor ER -