TY - JOUR T1 - Waves of sumoylation support transcription dynamics during adipocyte differentiation JF - bioRxiv DO - 10.1101/2021.02.20.432084 SP - 2021.02.20.432084 AU - Xu Zhao AU - Ivo A. Hendriks AU - Stéphanie Le Gras AU - Tao Ye AU - P Aurélie Nguéa AU - Guro Flor Lien AU - Lucia Ramos-Alonso AU - Arne Klungland AU - Bernard Jost AU - Jorrit M. Enserink AU - Michael L. Nielsen AU - Pierre Chymkowitch Y1 - 2021/01/01 UR - http://biorxiv.org/content/early/2021/02/20/2021.02.20.432084.abstract N2 - Tight control of gene expression networks required for adipose tissue formation and plasticity is essential for adaptation to energy needs and environmental cues. However, little is known about the mechanisms that orchestrate the dramatic transcriptional changes leading to adipocyte differentiation. We investigated the regulation of nascent transcription by SUMO during adipocyte differentiation using SLAMseq and ChIPseq. We discovered that SUMO has a dual function in differentiation; it supports the initial downregulation of pre-adipocyte-specific genes, while it promotes the establishment of the mature adipocyte transcriptional program. By characterizing sumoylome dynamics in differentiating adipocytes by mass spectrometry, we found that sumoylation of chromatin modifiers and specific transcription factors like Pparγ/RXR promotes the transcription of adipogenic genes. Our data demonstrate that the sumoylation pathway coordinates the rewiring of transcriptional networks required for formation of functional adipocytes.Competing Interest StatementThe authors have declared no competing interest. ER -