PT  - JOURNAL ARTICLE
AU  - Xu Zhao
AU  - Ivo A. Hendriks
AU  - Stéphanie Le Gras
AU  - Tao Ye
AU  - P Aurélie Nguéa
AU  - Guro Flor Lien
AU  - Lucia Ramos-Alonso
AU  - Arne Klungland
AU  - Bernard Jost
AU  - Jorrit M. Enserink
AU  - Michael L. Nielsen
AU  - Pierre Chymkowitch
TI  - Waves of sumoylation support transcription dynamics during adipocyte differentiation
AID  - 10.1101/2021.02.20.432084
DP  - 2021 Jan 01
TA  - bioRxiv
PG  - 2021.02.20.432084
4099  - http://biorxiv.org/content/early/2021/02/20/2021.02.20.432084.short
4100  - http://biorxiv.org/content/early/2021/02/20/2021.02.20.432084.full
AB  - Tight control of gene expression networks required for adipose tissue formation and plasticity is essential for adaptation to energy needs and environmental cues. However, little is known about the mechanisms that orchestrate the dramatic transcriptional changes leading to adipocyte differentiation. We investigated the regulation of nascent transcription by SUMO during adipocyte differentiation using SLAMseq and ChIPseq. We discovered that SUMO has a dual function in differentiation; it supports the initial downregulation of pre-adipocyte-specific genes, while it promotes the establishment of the mature adipocyte transcriptional program. By characterizing sumoylome dynamics in differentiating adipocytes by mass spectrometry, we found that sumoylation of chromatin modifiers and specific transcription factors like Pparγ/RXR promotes the transcription of adipogenic genes. Our data demonstrate that the sumoylation pathway coordinates the rewiring of transcriptional networks required for formation of functional adipocytes.Competing Interest StatementThe authors have declared no competing interest.