RT Journal Article
SR Electronic
T1 A Neuroendocrine Modulation to Sustain <em>C. elegans</em> Forward Locomotion
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 065235
DO 10.1101/065235
A1 Maria A. Lim
A1 Jyothsna Chitturi
A1 Valeriya Laskova
A1 Jun Meng
A1 Daniel Findeis
A1 Anne Wiekenberg
A1 Ben Mulcahy
A1 Linjiao Luo
A1 Yan Li
A1 Yangning Lu
A1 Wesley Hung
A1 Yixin Qu
A1 Chi-Yip Ho
A1 Douglas Holmyard
A1 Rebecca McWhirter
A1 Ni Ji
A1 Aravinthan D. T. Samuel
A1 David M. Miller III
A1 Ralf Schnabel
A1 John A. Calarco
A1 Mei Zhen
YR 2016
UL http://biorxiv.org/content/early/2016/07/23/065235.abstract
AB HighlightsRID is a specialized peptidergic neuron.UNC-39/Six/SO governs RID neurogenesis.Subtractive RNA profiling uncovers FLP, INS and NLP neuropeptides in RID.RID sustains C. elegans forward locomotion in part through FLP-14.Summary Neuromodulators shape neural circuit dynamics. Combining electron microscopy, genetics, transcriptome profiling, calcium imaging and optogenetics, we discovered a peptidergic neuron that sustains C. elegans forward movement. The Six/SO-family homeobox transcription factor UNC-39 governs lineage-specific neurogenesis to give rise to a neuron called RID. RID bears hallmarks of a specialized neuroendocrine: it harbors near-exclusive dense core vesicle clusters along the axon, and expresses multiple neuropeptides, including FLP-14. RID activity increases during forward movement. Ablating RID reduces the sustainability of forward movement, a phenotype partially recapitulated by removing FLP-14. Activating RID prolongs forward movement, an effect that requires FLP-14. In summary, RID is a specialized endocrine neuron that promotes C. elegans forward motor state in part through a neuropeptide FLP-14.