PT  - JOURNAL ARTICLE
AU  - Jiaze Tang
AU  - Anji Ju
AU  - Boya Li
AU  - Shaosen Zhang
AU  - Yuanchao Gong
AU  - Boyuan Ma
AU  - Yi Jiang
AU  - Hongyi Liu
AU  - Yan Fu
AU  - Yongzhang Luo
TI  - Young and Undamaged rMSA Improves the Longevity of Mice
AID  - 10.1101/2021.02.21.432135
DP  - 2021 Jan 01
TA  - bioRxiv
PG  - 2021.02.21.432135
4099  - http://biorxiv.org/content/early/2021/02/21/2021.02.21.432135.short
4100  - http://biorxiv.org/content/early/2021/02/21/2021.02.21.432135.full
AB  - Improvement of longevity is an eternal dream of human beings. Here we report that a single protein recombinant mouse serum albumin (rMSA) improved the lifespan and healthspan of C57BL/6N mice. The median lifespan extensions were 17.6% for female and 20.3% for male, respectively. The grip strength of rMSA-treated female and male mice increased by 29.6% and 17.4%, respectively. Meanwhile, the percentage of successful escape increased 23.0% in rMSA-treated male mice using the Barnes Maze test. The rMSA used in this study is young and almost undamaged. We define the concept “young and undamaged” to any protein without any unnecessary modifications by four parameters: intact free thiol (if any), no advanced glycation end-product, no carbonylation, and no homocysteinylation. Here “young and undamaged” rMSA is much younger and less damaged than the endogenous serum albumin from young mice at 1.5 months of age. We predict that young and undamaged proteins altogether can further improve the longevity.Competing Interest StatementThe authors have declared no competing interest.AbbreviationsHSAhuman serum albumin.AGEadvanced glycation end-product.Hcyhomocysteine.rMSArecombinant mouse serum albumin.p-tauphosphorylated microtubule-associated protein tau.MYH1myosin heavy chain I.α-SMAα-smooth muscle actin.COL1A1collagen I.HCPhost cell proteins.qRT-PCRquantitative RT-PCR.ELISAenzyme-linked immunosorbent assay.GAPDHglyceraldehyde 3-phosphate dehydrogenase.DAPI4′,6-diamidino-2-phenylindole.DTNB5, 5’-Dithiobis-(2-nitrobenzoic acid).Q-TOFQuadrupole-Time of Flight.K-S testKolmogorov-Smirnov test.