RT Journal Article
SR Electronic
T1 Young and Undamaged rMSA Improves the Longevity of Mice
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 2021.02.21.432135
DO 10.1101/2021.02.21.432135
A1 Jiaze Tang
A1 Anji Ju
A1 Boya Li
A1 Shaosen Zhang
A1 Yuanchao Gong
A1 Boyuan Ma
A1 Yi Jiang
A1 Hongyi Liu
A1 Yan Fu
A1 Yongzhang Luo
YR 2021
UL http://biorxiv.org/content/early/2021/02/21/2021.02.21.432135.abstract
AB Improvement of longevity is an eternal dream of human beings. Here we report that a single protein recombinant mouse serum albumin (rMSA) improved the lifespan and healthspan of C57BL/6N mice. The median lifespan extensions were 17.6% for female and 20.3% for male, respectively. The grip strength of rMSA-treated female and male mice increased by 29.6% and 17.4%, respectively. Meanwhile, the percentage of successful escape increased 23.0% in rMSA-treated male mice using the Barnes Maze test. The rMSA used in this study is young and almost undamaged. We define the concept “young and undamaged” to any protein without any unnecessary modifications by four parameters: intact free thiol (if any), no advanced glycation end-product, no carbonylation, and no homocysteinylation. Here “young and undamaged” rMSA is much younger and less damaged than the endogenous serum albumin from young mice at 1.5 months of age. We predict that young and undamaged proteins altogether can further improve the longevity.Competing Interest StatementThe authors have declared no competing interest.AbbreviationsHSAhuman serum albumin.AGEadvanced glycation end-product.Hcyhomocysteine.rMSArecombinant mouse serum albumin.p-tauphosphorylated microtubule-associated protein tau.MYH1myosin heavy chain I.α-SMAα-smooth muscle actin.COL1A1collagen I.HCPhost cell proteins.qRT-PCRquantitative RT-PCR.ELISAenzyme-linked immunosorbent assay.GAPDHglyceraldehyde 3-phosphate dehydrogenase.DAPI4′,6-diamidino-2-phenylindole.DTNB5, 5’-Dithiobis-(2-nitrobenzoic acid).Q-TOFQuadrupole-Time of Flight.K-S testKolmogorov-Smirnov test.