RT Journal Article
SR Electronic
T1 Monitoring oxidative inflammatory processes in live cells and tissue with Hypocrates, a genetically encoded biosensor for hypochlorite
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 2021.02.22.432222
DO 10.1101/2021.02.22.432222
A1 Alexander I. Kostyuk
A1 Maria-Armineh Tossounian
A1 Anastasiya S. Panova
A1 Marion Thauvin
A1 Khadija Wahni
A1 Inge Van Molle
A1 Roman I. Raevskii
A1 Mikhail S. Baranov
A1 Sophie Vriz
A1 Joris Messens
A1 Dmitry S. Bilan
A1 Vsevolod V. Belousov
YR 2021
UL http://biorxiv.org/content/early/2021/02/22/2021.02.22.432222.abstract
AB Hypochlorous acid, an aggressive oxidant, is important in immune defense against pathogens. The current lack of tools to monitor the dynamics of hypochlorous acid in live cells and tissue hinders a better understanding of inflammatory processes. We engineered a genetically encoded biosensor, Hypocrates, for the visualization of hypochlorous acid. Hypocrates consists of a circularly permuted yellow fluorescent protein integrated into the structure of the transcription repressor NemR from E. coli. We determined sensitivity, selectivity, reaction rates, and the X-ray structure of this ratiometric redox biosensor, and tested the response of Hypocrates in HeLa Kyoto cells at varying hypochlorite concentrations. By combining Hypocrates with the biosensor HyperRed, we visualized the dynamics of hypochlorous acid and hydrogen peroxide in a zebrafish tail fin injury model.Competing Interest StatementThe authors have declared no competing interest.