RT Journal Article
SR Electronic
T1 Multiple PDZ Domain Protein Maintains Patterning of the Apical Cytoskeleton in Sensory Hair Cells
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 2021.02.20.432099
DO 10.1101/2021.02.20.432099
A1 Jarysta, Amandine
A1 Tarchini, Basile
YR 2021
UL http://biorxiv.org/content/early/2021/02/22/2021.02.20.432099.abstract
AB Sound transduction occurs in the hair bundle, the apical compartment of sensory hair cells in the inner ear. The hair bundle is formed of stereocilia aligned in rows of graded heights. It was previously shown that the GNAI-GPSM2 complex is part of a developmental blueprint that defines the polarized organization of the apical cytoskeleton in hair cells, including stereocilia distribution and elongation. Here we report a novel and critical role for Multiple PDZ domain (MPDZ) protein during apical hair cell morphogenesis. We show that MPDZ is enriched at the hair cell apical membrane, and required there to maintain the proper segregation of apical blueprints proteins, including GNAI-GPSM2. Loss of the blueprint coincides with misaligned stereocilia in Mpdz mutants, and results in permanently misshapen hair bundles. Graded molecular and structural defects along the cochlea can explain the profile of hearing loss in Mpdz mutants, where deficits are most severe at high frequencies.Competing Interest StatementThe authors have declared no competing interest.