RT Journal Article
SR Electronic
T1 Pancreas patch-seq links physiologic dysfunction in diabetes to single-cell transcriptomic phenotypes
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 555110
DO 10.1101/555110
A1 Camunas-Soler, Joan
A1 Dai, Xiaoqing
A1 Hang, Yan
A1 Bautista, Austin
A1 Lyon, James
A1 Suzuki, Kunimasa
A1 Kim, Seung K
A1 Quake, Stephen R
A1 MacDonald, Patrick E
YR 2019
UL http://biorxiv.org/content/early/2019/02/20/555110.abstract
AB Pancreatic islet cells regulate glucose homeostasis through insulin and glucagon secretion; dysfunction of these cells leads to severe diseases like diabetes. Prior single-cell transcriptome studies have shown heterogeneous gene expression in major islet cell-types; however it remains challenging to reconcile this transcriptomic heterogeneity with observed islet cell functional variation. Here we achieved electrophysiological profiling and single-cell RNA sequencing in the same islet cell (pancreas patch-seq) thereby linking transcriptomic phenotypes to physiologic properties. We collected 1,369 cells from the pancreas of donors with or without diabetes and assessed function-gene expression networks. We identified a set of genes and pathways that drive functional heterogeneity in β-cells and used these to predict β-cell electrophysiology. We also report specific transcriptional programs that correlate with dysfunction in type 2 diabetes (T2D) and extend this approach to cryopreserved cells from donors with type 1 diabetes (T1D), generating a valuable resource for understanding islet cell heterogeneity in health and disease.Key findingsPancreas patch-seq provides a single-cell survey of function-transcriptome pairing in 1,369 islet cells from donors with and without diabetesExpression of a specific subset of genes predicts β-cell electrophysiology in transcriptome-function networks.Compromised β-cell function in T2D correlates with altered ETV1 expression and inflammatory pathwaysFunctional heterogeneity in α-cells maps to ER stress and islet lineage markersApplication of patch-seq to cells from rare cryopreserved islets from donors with T1D