RT Journal Article
SR Electronic
T1 A Comparison of Performance for Different SARS-Cov-2 Sequencing Protocols
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 2021.03.01.433428
DO 10.1101/2021.03.01.433428
A1 Juanjo Bermúdez
YR 2021
UL http://biorxiv.org/content/early/2021/03/01/2021.03.01.433428.abstract
AB SARS-Cov-2 genome sequencing has been identified as a fundamental tool for fighting the COVID-19 pandemic. It is used, for example, for identifying new variants of the virus and for elaborating phylogenetic trees that help to trace the spread of the virus. In the present study we provide a comprehensive comparison between the quality of the assemblies obtained from different sequencing protocols. We demonstrate how some protocols actively promoted by different high-level administrations are inefficient and how less-used alternative protocols show a significant increased performance. This increase of performance could lead to cheaper sequencing protocols and therefore to a more convenient escalation of the sequencing efforts around the world.Competing Interest StatementI am the developer and the owner of all the rights of the s-aligner software.