TY - JOUR T1 - Negative Feedback Facilitates Temperature Robustness in Biomolecular Circuit Dynamics JF - bioRxiv DO - 10.1101/007385 SP - 007385 AU - Shaunak Sen AU - Richard M. Murray Y1 - 2014/01/01 UR - http://biorxiv.org/content/early/2014/07/22/007385.abstract N2 - Temporal dynamics in many biomolecular circuits can change with temperature because of the temperature dependence of underlying reaction rate parameters. It is generally unclear what circuit mechanisms can inherently facilitate robustness in the dynamics to variations in temperature. Here, we address this issue using a combination of mathematical models and experimental measurements in a cell-free transcription-translation system. We find that negative transcriptional feedback can reduce the effect of temperature variation on circuit dynamics. Further, we find that effective negative feedback due to first-order degradation mechanisms can also enable such a temperature robustness effect. Finally, we estimate temperature dependence of key parameters mediating such negative feedback mechanisms. These results should be useful in the design of temperature robust circuit dynamics. ER -