PT  - JOURNAL ARTICLE
AU  - Kosuke Kuwabara
AU  - Issei Harada
AU  - Yuma Matsuzawa
AU  - Tohru Ariizumi
AU  - Kenta Shirasawa
TI  - Organelle genome assembly uncovers the dynamic genome reorganization and cytoplasmic male sterility associated genes in tomato
AID  - 10.1101/2021.03.03.433741
DP  - 2021 Jan 01
TA  - bioRxiv
PG  - 2021.03.03.433741
4099  - http://biorxiv.org/content/early/2021/03/04/2021.03.03.433741.short
4100  - http://biorxiv.org/content/early/2021/03/04/2021.03.03.433741.full
AB  - To identify cytoplasmic male sterility (CMS)-associated genes in tomato, we determined the genome sequences of mitochondria and chloroplasts in three CMS tomato lines derived from independent asymmetric cell fusions, their nuclear and cytoplasmic donors, and male fertile weedy cultivated tomato and wild relatives. The structures of the CMS mitochondrial genomes were highly divergent from those of the nuclear and cytoplasmic donors, and genes of the donors were mixed up in these genomes. On the other hand, the structures of CMS chloroplast genomes were moderately conserved across the donors, but CMS chloroplast genes were unexpectedly likely derived from the nuclear donors. Comparative analysis of the structures and contents of organelle genes and transcriptome analysis identified three genes that were uniquely present in the CMS lines, but not in the donor or fertile lines. RNA sequencing analysis indicated that these three genes transcriptionally expressed in anther, two of which were also expressed in pollen. They could be potential candidates for CMS-associated genes. This study suggests that organelle reorganization mechanisms after cell fusion events differ between mitochondria and chloroplasts, and provides insight into the development of new F1 hybrid breeding programs employing the CMS system in tomato.Competing Interest StatementYM is an employee of TOKITA Seed Co. LTD. All other authors declare no competing interests.