%0 Journal Article %A Yusuke Nasu %A Ciaran Murphy-Royal %A Yurong Wen %A Jordan Haidey %A M. Rosana S. Molina %A Abhi Aggarwal %A Shuce Zhang %A Yuki Kamijo %A Marie-Eve Paquet %A Kaspar Podgorski %A Mikhail Drobizhev %A Jaideep S. Bains %A M. Joanne Lemieux %A Grant R. Gordon %A Robert E. Campbell %T A genetically encoded fluorescent biosensor for extracellular L-lactate %D 2021 %R 10.1101/2021.03.05.434048 %J bioRxiv %P 2021.03.05.434048 %X To enable investigations of the emerging roles of cell-to-cell shuttling of L-lactate, we have developed an intensiometric green fluorescent genetically encoded biosensor for extracellular L-lactate. We demonstrate that this biosensor, designated eLACCO1.1, enables minimally invasive cellular resolution imaging of extracellular L-lactate in cultured mammalian cells and brain tissue.Competing Interest StatementThe authors have declared no competing interest. %U https://www.biorxiv.org/content/biorxiv/early/2021/03/06/2021.03.05.434048.full.pdf