TY - JOUR T1 - Anaerobic microbiota facilitate <em>P. aeruginosa</em> access to the airway epithelium in a novel co-culture model of colonization JF - bioRxiv DO - 10.1101/2021.03.05.433759 SP - 2021.03.05.433759 AU - Patrick J. Moore AU - Talia D. Wiggen AU - Leslie A. Kent AU - Sabrina J. Arif AU - Sarah K. Lucas AU - Scott M. O’Grady AU - Ryan C. Hunter Y1 - 2021/01/01 UR - http://biorxiv.org/content/early/2021/03/06/2021.03.05.433759.abstract N2 - The role(s) of anaerobic microbiota in chronic airway disease are poorly understood due to inherent limitations of existing laboratory models. To address this knowledge gap, we use a dual oxic-anoxic co-culture approach that maintains an oxygen-limited apical epithelial microenvironment while host cells are oxygenated basolaterally. Reduced oxygen culture did not alter the physiology or gene expression of Calu-3 cells but supported anaerobe-epithelial interactions for 24h without affecting bacterial or host cell viability. Anaerobe challenge led to increased expression of inflammatory marker genes and compromised integrity of apical mucins, leading to our hypothesis that anaerobe-host interactions prime the airways for chronic infection. Indeed, anaerobe pre-treatment of Calu-3 cells led to an increase in Pseudomonas aeruginosa colonization. This model system offers new insight into anaerobe-host interactions in airway disease pathophysiology and motivates further study of the lung, gut, and oral cavity, where etiological roles of anaerobes have been proposed but specific pathogenic mechanisms remain unclear.Competing Interest StatementThe authors have declared no competing interest. ER -