PT  - JOURNAL ARTICLE
AU  - Yasuhiro Shishikura
AU  - Katsuyuki Tokinoya
AU  - Yuichi Aita
AU  - Nanami Sekine
AU  - Takehito Sugasawa
AU  - Yasuko Yoshida
AU  - Keisei Kosaki
AU  - Shota Kumamoto
AU  - Keisuke Ishikura
AU  - Tomoaki Kuji
AU  - Yasushi Kawakami
AU  - Yoshiharu Nabekura
AU  - Seiji Maeda
AU  - Kazuhiro Takekoshi
TI  - The dynamics of cell-free DNA from urine and blood after a full marathon
AID  - 10.1101/2021.03.06.434188
DP  - 2021 Jan 01
TA  - bioRxiv
PG  - 2021.03.06.434188
4099  - http://biorxiv.org/content/early/2021/03/08/2021.03.06.434188.short
4100  - http://biorxiv.org/content/early/2021/03/08/2021.03.06.434188.full
AB  - Purpose Cell-free DNA (cfDNA) has been investigated as a minimally invasive biomarker for many diseases, particularly cancer. An increase in cfDNA has been observed during exercise. Neutrophil extracellular traps (NETs) may be the origin of cfDNA in response to acute exercise, but the mechanisms of generation of cfDNA during exercise remain unclear. In this study we investigated the dynamics of serum and urinary cfDNA levels and determined the relevance of other biomarkers to serum and urinary cfDNA levels and fragment size after a full marathon.Methods Samples were collected from 23 healthy male subjects. Blood and urine samples were collected before and immediately, two hours, and one day after the full marathon. The measurements included serum and urinary cfDNA, creatine kinase, myoglobin, creatinine, white blood cells, platelets, and lactoferrin from blood, and amylase, albumin, and creatinine from urine.Results Serum and urinary cfDNA levels increased after a full marathon. Creatine kinase, myoglobin, and creatinine in blood, and albumin and creatinine in urine also increased significantly after a full marathon. Serum cfDNA showed peak values about 180 bp after the full marathon. Values over 1000 bp were present at two hours post-marathon. Urinary cfDNA showed peak values from 35 bp to 50 bp after the full marathon. Values over 1000 bp appeared at Immediately and two hours post marathon.Conclusion This study revealed that both serum and urinary cfDNA levels transiently increased after a full marathon. In addition, these cfDNA fragment varied in size.Competing Interest StatementThe authors have declared no competing interest.